Service Centre category(ies)
Cell Analysis / Flow Cytometry
Biobanks / Clinical Samples / Histology / Pathology
Microscopy
Molecular fractionation, isolation, purification and production
Genomics / Gene Expression Analysis / DNA Sequencing / DNA Synthesis / Real-Time PCR / Genotyping / Microarray
Proteomics / Peptide Synthesis
Description of the Service Centre
This facility is dedicated to the analysis of gene expression at DNA, RNA and protein level, routinely performing a multitude of biochemical and molecular biology techniques using standard equipment for electrophoresis, western blot, northern bot, as well absorbance plate reader, and gel, membrane and X-ray film imaging (Chemidoc MP and XRS; Bio-Rad).
Service Centre web address
http://imed.ulisboa.pt/research/resources-services/cell-biology-laboratory-2/
Contact person's name
Rui Castro
Contact person's e-mail
ruieduardocastro@ff.ulisboa.pt
Contact person's telephone
351217946400
Last edited on
2014-09-30 16:58:15
Service
Cell Biology
Available for external use?
Yes
Comments
It is also equipped with end-point (Bio-Rad and Thermo) and real time PCR (Applied Biosystems 7300 Real-Time PCR System) thermocyclers, and a Guava easycyte 5HT benchtop flow cytometer for high-throughput cell analysis in 96-well format. Additionally, there is a stopped-flow (HiTech Scientific) and two Patch-clamp setups (Axon Instruments), used for kinetic analyses of ion channel activity in cells. Fluorescence and bright field microscopes (Zeiss) with dedicated cameras (Leica) and acquisition software are available.
Equipment
Standard western and northern blot equipment (Bio-Rad)
Service type: self-service or full-service?
Full-service
Equipment
Chemidoc MP and XRS (Bio-Rad)
Service type: self-service or full-service?
Full-service
Equipment
Glomax multiplate reader (Promega)
Service type: self-service or full-service?
Full-service
Equipment
7300 Real-Time PCR System (Applied Biosystems)
Service type: self-service or full-service?
Full-service
Equipment
Termocycler Unocycler (VWR)
Service type: self-service or full-service?
Full-service
Equipment
Guava easycyte 5HT (Millipore)
Service type: self-service or full-service?
Full-service
Equipment
Stopped-flow (HiTech Scientific)
Service type: self-service or full-service?
Full-service
Equipment
Patch-clamp setups (Axon Instruments)
Service type: self-service or full-service?
Full-service
Equipment
Microscope Axio Scope.A1 coupled with a AxioCam HR (Zeiss)
Service type: self-service or full-service?
Full-service
Equipment
Microscope Axio Scope (Zeiss) + Leica DFC 490 (Leica)
Service type: self-service or full-service?
Full-service