Service Centre category(ies)
Cell Analysis / Flow Cytometry
Cell Culture and Manipulation
Molecular fractionation, isolation, purification and production
Description of the Service Centre
Rodon Biologics is a service provider company with more than 15 years experience in protein recombinant production andprocess development. Rodon is a spin-off of Biotecnol, a company operating in Portugal since 1996 dedicated to biological development. Rodon services include: protein production, analytical characterization, cell-based assays, cell line and process development. Rodon is customer-centric and guarantees that the projects are executed under a quality system.
Service Centre web address
Contact person's name
Ana Rita Ricardo
Contact person's e-mail
arr@rodonbiologics.com
Contact person's telephone
351214220520
Last edited on
2018-02-27 16:02:50
Service
Analytical characterization
Available for external use?
Yes
Comments
At Rodon we have the experience to develop and execute the required analytical procedures to assure product and process quality. Tests are designed in consideration of the relevant Pharmacopoeia (EU and US monographs) and regulatory guidelines (ICH, FDA and EMEA) and considering GMP practices.
Service
Cell line development
Available for external use?
Yes
Comments
Rodon’s cell line development activities are performed as a stand-alone service or are fully integrated into a process development program. Rodon can create vectors to express product using CHO platform. Protein yields of grams-per-liter range have been achieved using this system in chemically defined, animal-, component-free medium. This procedure is based on a simple workflow which allows obtaining a high-producing single cell clone in 13-18 weeks. Moreover, once a high producing pool is generated, the quality of the material is evaluated and it can be used to produce gram-per-liter quantities of material in 8-11 weeks.
Service
Cell-based assays
Available for external use?
Yes
Comments
Bioassays are one of the few analytical tools where native proteins can be characterized in vitro, under physiological conditions. They are essential in assuring integrity and functionality of a biological product. Our expertise includes the setting of binding, proliferation, cytotoxic and viral assays. At Rodon these types of bioassays are implemented and carried out on a routine basis in biosafety level 2 laboratory facilities, against suitable controls, and wherever possible International Reference Standards.
Service
Process development
Available for external use?
Yes
Comments
Rodon has extensive experience with both microbial (E.coli, yeast) and mammalian cell culture derived proteins. Using our proprietary and in-licensed technology for expression proteins are rapidly screened in different constructs, to identify the most suitable system, using tightly-regulated inducible promoters for microbial cells. For each protein cloned, a careful development program is carried out in order to study in detail, optimal protocols based on the protein characteristics, process steps and conditions. The process optimization is performed in parallel with the DSP process development in order to obtain a global high yielding and high quality process. Process development programme is also cost effective, robust and transferable driven.
Service
Recombinant protein production
Available for external use?
Yes
Comments
Rodon\'s custom recombinant protein services comprise a portfolio of recombinant protein expression systems that include bacteria, yeast and mammalian cells. Our company offers a full spectrum of recombinant protein purification methods to achieve the ideal level of purity for research purposes. With customized solutions and flexible production scale we work in partnership with our clients to ensure efficient and high quality recombinant protein production, process development and scale-up production of biologics.